Autofluoresence in yeast?

Fergus Doherty Fergus.Doherty at
Sun Dec 14 23:03:27 EST 1997

Is there are problem with (green) autofluoresence in S. cerevisiae? I have
high backgrounds using mouse primary, biotin anti-mouse and Sigma
FITC-Extravidin. Or is the avidin-biotin system giving a background due to
endogenous biotin.  Fixation and permeablisation as per standard protocols
(e.g. Pringle, Methods in Enzymol, 194).


Fergus Doherty,
Dept Biochemistry,
Nottingham University,

Fergus.Doherty at
0115 970 9366 (74-41366 internal)

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