Library Amplification Problems

Thu Feb 6 18:58:10 EST 1997

Hi yeast users,

I am trying to amplify a cDNA expression library (Clontech) which is 
cloned into pACT and transformed into the E. coli strain BNN132. The 
problem I'm having is getting the bacteria to lyse properly. I have been 
using normal maxi-prep solutions, and the yields of DNA have been 
terrible. If anybody has had the same problem and found a method for 
improving DNA yeilds, some suggestions would be much appreciated.


Glenn Samwell.

Cancer Research Unit,
Newcastle, Australia.

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