Library Amplification Problems

Ian Burbulis harley at vt.edu
Thu Feb 6 21:00:19 EST 1997


Glenn Samwell had a question about library amplification:

Use an e.coli strain called DH10B. This strain is made for amplifing
libraries, and works great. With electroporation this strain has a
transformation efficiency of >10E10. It also has an endA1 mutation which
improves lysis. It is sold commercialy by gibco/life tech. Or I'm sure
somebody around may have some to spare. Your return address is improper and
will not recieve out-going e-mails.

Ian 
Va Tech
harley at vt.edu




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