FOA problems

Todd Hryciw toddh at
Mon Feb 10 17:25:18 EST 1997

for some reason when I screen for the URA "pop out" on SD-ura plates I get
growth, suggesting the cells are still URA+, but the same cells cultured
in SD-ura liquid don't grow.  I've taken those cells which don't grow in
liquid to be His recombinants.  I don't know if this is truly valid
though.  Any comments?

Todd Hryciw
Dept. of Microbiology, U. of Saskatchewan

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