FOA problems

Todd Hryciw toddh at duke.usask.ca
Mon Feb 10 17:25:18 EST 1997


Hi,
for some reason when I screen for the URA "pop out" on SD-ura plates I get
growth, suggesting the cells are still URA+, but the same cells cultured
in SD-ura liquid don't grow.  I've taken those cells which don't grow in
liquid to be His recombinants.  I don't know if this is truly valid
though.  Any comments?

Todd Hryciw
Dept. of Microbiology, U. of Saskatchewan
Canada





More information about the Yeast mailing list