Yeast two-hybrid system

Anne Phelan a.phelan at scorch.vir.gla.ac.uk
Thu Feb 13 06:04:03 EST 1997


I have been trying to grow HF7C yeast cells that have been transformed with my 
bait plasmid in SD-TRP medium.  Following overnight culture the cells 
aggregate at the bottom of the universal.  Has anyone else had this problem?  
Any ideas why?

Many thanks


Sarah Wadd
Insitute of Virology
Glasgow



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