unstable yeast DNA in E. coli

betina at univ-lyon1.fr betina at univ-lyon1.fr
Wed Feb 19 10:45:08 EST 1997


Hi!
I have isolated plasmid containing yeast DNA that complements the 
mutation I'm interested in. Unfortunatelly, this clone is very unstable
in E. coli and I'm loosing the whole fragment of interest. I have tried
several "shure" rec- strains (including Sure), but this does not help. 
Has anyone experienced something similar? I would welcome any suggestions
or tips how to overcome this unstability. Thanks.
Svato Betina





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