Sonication of yeast for SDSPAGE?
Achim Recktenwald, PhD
achim at ibex.ca
Wed Feb 26 15:57:43 EST 1997
Fergus Doherty wrote:
> Would sonication of yeast in SDSPAGE sample buffer serve to break them up
> and solublise protein for SDSPAGE? This seems easier than glass beads.
> Fergus Doherty
> Nottingham University UK
The harsh conditions during sonication will probably oxidize your
beta-mercaptoethanol or DTT in the sample buffer.
Additionally, sonication of yeast cells is often not very successful.
The very flexible cell walls just resonate with the frequency of the
sonicator. Therefore, to increase the yield of the energy transfer to
the cells, glass beads are added.
More information about the Yeast