Sonication of yeast for SDSPAGE?

Achim Recktenwald, PhD achim at ibex.ca
Wed Feb 26 15:57:43 EST 1997


Fergus Doherty wrote:
> 
> Would sonication of yeast in SDSPAGE sample buffer serve to break them up
> and solublise protein for SDSPAGE?  This seems easier than glass beads.
> Fergus Doherty
> Nottingham University UK


The harsh conditions during sonication will probably oxidize your
beta-mercaptoethanol or DTT in the sample buffer. 
Additionally, sonication of yeast cells is often not very successful.
The very flexible cell walls just resonate with the frequency of the
sonicator. Therefore, to increase the yield of the energy transfer to
the cells, glass beads are added.

Achim



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