Sequential gene disruption

Chuck Staben staben at pop.uky.edu
Thu Jan 2 12:46:22 EST 1997


I had planned to use sequential gene disruption based on the
lox/kanamycin vectors to disrupt a set of transporter genes in various
combinations.

Unfortunately, disruption of these genes significantly increases
susceptibility to G418!  Makes selecting the disruptant probably
impossible.

Other than the hisG-URA3 cassette of Fonzi, are there other sequential
disruption vectors available?  Such as a URA3/lox cassette?



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