cell sorting live yeast
cosentino at medcor.mcgill.ca
Fri Jan 10 11:24:35 EST 1997
In article <32D514EE.7B7B at ryburn.swmed.edu>, Bev Rothermel
<rothermel at ryburn.swmed.edu> wrote:
> I'm looking for information on flow cytometry cell sorting of live
> unfixed yeast cells. Will it work, and does anyone have a good method?
> I want to use GFP as the cell marker and be able to grow up the cells
> after sorting for multiple rounds. The only methods I've seen call for
> fixing the cells prior to FACS.
> Bev Rothermel
> rothermel at ryburn.swmed.edu
Try Niedenthal et al., Yeast (1996) 12, 773-786.
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