cell sorting live yeast

Greg Cosentino cosentino at medcor.mcgill.ca
Fri Jan 10 11:24:35 EST 1997


In article <32D514EE.7B7B at ryburn.swmed.edu>, Bev Rothermel
<rothermel at ryburn.swmed.edu> wrote:

> I'm looking for information on flow cytometry cell sorting of live 
> unfixed yeast cells.  Will it work, and does anyone have a good method?  
> I want to use GFP as the cell marker and be able to grow up the cells 
> after sorting for multiple rounds.  The only methods I've seen call for 
> fixing the cells prior to FACS. 
> 
> Thanks,
> 
> Bev Rothermel
> rothermel at ryburn.swmed.edu

Hi,

Try Niedenthal et al., Yeast (1996) 12, 773-786.

Good Luck
Greg



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