How I get Mitos!!!

Michael Lichten lichten at helix.nih.gov
Mon Jan 13 09:43:26 EST 1997


In article <1997Jan10.172910 at crick.rz-berlin.mpg.de>,
jaensch at mpimg-berlin-dahlem.mpg.de wrote:

> I try to get yeast mitochondria. The removal of the cell wall with zymolyase
> doesnt work very well. Is the activity of the enzym depending on the growth
> conditions of the yeast cell? Which factors are important to optimize this
> step?

This may sound trivial, but make sure that the pH of your spheroplasting
buffer is below pH 8.0.  The enzyme doesn't work well above this.  pH 7.5
is what we use routinely.

-- 
Michael Lichten
lichten at helix.nih.gov



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