Pombe

Rachael Buchnik rbuchnik at MAIL.NCIFCRF.GOV
Thu Jan 16 16:45:22 EST 1997


Hello!
Can anyone explain why my DNA isolated from strains on FOA plates won't cut 
with HindIII (despite repeated attempts at purification)? The identical 
strains grown on YEA or -ura plates give fine, cuttable DNA.  The strains 
express low levels of ura, and thus grow about equally badly on FOA and ura, 
yielding similar ammounts of DNA.  Any ideas?  




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