gst fusions in yeast

hannes klump hannes at
Mon Jan 27 20:54:04 EST 1997

Hi yeasters!
Does anyone know, whether it is possible to use GST-fusions for
expression and purification via glutathion columns.
The main problem, I guess, is the binding of glutation in the yeast cell
to the GST, but this should happen to a minor proportion of the whole
lot of expressed recombinant protein, anyway;
Has anyone tried it yet?
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