Preparation of yeast nuclear extracts

Malcolm White mfwhite at bad.dundee.ac.uk
Thu Jan 30 05:10:18 EST 1997


Does anyone have a reccomendation for a "quick and dirty" method to enrich
for nuclear proteins from a S.cerevisiae extract. I am really interested
in getting rid of the bulk of the cytosolic proteins before starting
purification of a nuclear protein.

Any advice or comments would be greatly appreciated

Malcolm White

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__________________________________________
Dr Malcolm F. White
Honorary Lecturer
University of Dundee
Scotland



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