Preparation of yeast nuclear extracts

Malcolm White mfwhite at
Thu Jan 30 05:10:18 EST 1997

Does anyone have a reccomendation for a "quick and dirty" method to enrich
for nuclear proteins from a S.cerevisiae extract. I am really interested
in getting rid of the bulk of the cytosolic proteins before starting
purification of a nuclear protein.

Any advice or comments would be greatly appreciated

Malcolm White

Dr Malcolm F. White
Honorary Lecturer
University of Dundee

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