Calcium precipitation!

* * at auckland.ac.nz
Wed Jul 2 19:12:59 EST 1997


In article <5pb1ol$5ok at mserv1.dl.ac.uk>, "Miguel A. Rodriguez Gabriel"
<MARODRIGUEZ at trasto.cbm.uam.es> wrote:

> Dear Yeasters,
> 
>         I am trying to prepare minimal medium (YNB 6.7 g/l; glucose 20
g/l) to 
> grow S. cerevisiae. I want to have a final concentration of 100 mM calcium in 
> this medium, and for this purpose I add CaCl2. My problem is that when I add 
> CaCl2, a white precipitate appears. 
>         Has any of you prepared this kind of medium? How did you solve this 
> PRECIPITATION problem?

Replace NH4SO4 with NH4Cl (3.5 g/L) and use YNB-nitrogen source and amino
acids (Difco). 
 
-Colin MacD.



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