2-hybrid - plasmid rescue

Harold E Smith hes at U.Arizona.EDU
Mon Jul 21 18:53:11 EST 1997


Dear Barry,
If you have primers that flank the insertion site of your cDNA
library, you might try PCR amplification of your insert from your yeast
transformants (aka whole cell PCR).  In addition to obtaining sequence
information, you could also reclone the PCR product into the two-hybrid
vector and retest for interaction with your bait.
Good luck,
Harold




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