2-hybrid - plasmid rescue

Donny Wong wong5 at login3.fas.harvard.edu
Thu Jul 24 09:48:25 EST 1997


does you bait vector have a cycloheximide sensitivity gene on it?  (CYH2).
if it does, you can grow your yeast on -leucine media containing
cycloheximide, which will result in the death of all clones harboring the
vector containing the CYH2 gene.  you should then have only the vector
bearing your prey cDNA.

hope this helps!
don



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