simple his tag expression vector

Damien hermand damien.hermand at fundp.ac.be
Sun Jun 1 10:16:11 EST 1997


In article (Dans l'article) <barnesl.1214711112A at uthscsa.edu>,
barnesl at UTHSCSA.EDU ("Larry D. Barnes") wrote (écrivait) :

> Jim Bone wrote:
> 
> >I am looking for a simple His tagged (amino terminus) vector that allows
> >expression by the T7 system.  If anyone knows of such a vector, please let
> >me know.
> 
> Ghosh and Lowenstein have constructed a multifunctional vector system for
> expression of his-tagged and native proteins via T7.  The reference is Gene
> 176, 249-255 (1996).
> 


pET15b from Novagen



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