Cell wall fluorescence

* * at auckland.ac.nz
Tue Jun 3 15:48:43 EST 1997


Hello,

I am trying to get fluorescence from yeast cells expressing GFP fused to a
cell membrane protein.  I appear to have quite high background
fluorescence in cells not expressing GFP.  Is this normal?  Can this be
reduced by removing the cell wall?

Any help much appreciated.

Thanks
Leigh Bradbury
lbradbury at auckland.ac.nz



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