3H-methionine labelling of yeast proteins?

Fergus Doherty Fergus.Doherty at nottingham.ac.uk
Thu Jun 12 09:04:45 EST 1997


I'd like to use a 3H labelled amino acid to label yeast cell proteins. 
Would 3H methionine be OK?  It is labelled in the terminal methyl group, so
this might be transferred to other molecules (ie methylation from adenosyl
methionine).  Anybody know if this will introduce significant label into
non-protein molecules.  Alternative labels (preferably not leucine)?

TIA

-- 
Fergus Doherty,
Dept Biochemistry,
Nottingham University,

Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)



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