Colony PCR (again)

[Aidan Weatherill]

I have been hunting for a reliably method of doing pcr from colonies, all
the protocols that I have come across require very fresh colonies, and
whilst this is generally not a problem I have recollections of seeing a
protocol that involves spherolplasting the cells and pcr'ing from them, as
I remember it claimed product from two month old colonies!!

Any help would be gratefully received.

regards
Aidan.
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                               Aidan weatherill

Yeast Genetics                             48 percy street
The Institute of Molecular Medicine        East Oxford
University of Oxford                       Oxford
The John Radcliffe                         OX4 3AF
Headington
Oxford
OX3 9DS

Tel: (01865) 222 405                       Tel:(01865) 241 505
                  E-mail: aweather at worf.molbiol.ox.ac.uk
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