S. pombe cell wall digestion

T.J. Young BMBTJY at leeds.ac.uk
Tue Jun 24 05:36:13 EST 1997


Hi,
I want to digest the cell walls from my S. pombe.  The protocols I have found 
use Novozyme sometimes together wil Zymolase.  The problem is Novozyme has 
some protease activity, which is a bad thing because I want to do some 
immunofluorescence on a plasma membrane protein I have expressed.  Does 
anyone know of any other enzymes, that have no protease activity, that I can 
use?  Can I use Zymolase alone?
Thanks in advance,
Tim :-)
Ph.D. Student
Leeds Uni.



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