Yeast maxi plasmid preps?

Kathy Baynton baynton at
Fri Mar 7 06:42:37 EST 1997

Dear yeast-handlers,

I was wondering if anyone knows if it is possible to produce maxi 
preps of plasmids from yeast that are relatively clean and in 
respectable quantities (ie. using 2mu-based origin plasmids) as is 
done in E. coli?  (ie CsCl purification, Wizard).  I am already aware 
of plasmid isolation preps from yeast (Hoffman and Winston I believe 
is the reference), but these preps are pretty dirty, the plasmid is 
not really "purified" and we always end up having to re-transform into 
E. coli in order to recover plasmids that are "clean enough" to do 
sequencing, cloning etc.  I am also aware of PCR-based sequening that 
can be done on yeast-isolated plasmids but for what we're thinking of 
doing, I'm not sure that this would be an appropriate method.  

Any information, advice...anything...would be tremendously 
appreciated!!  Thanks!!


More information about the Yeast mailing list