Ura3-52 deletion in Clontech strains

Hernan Espinoza espinoza at cgl.ucsf.edu
Mon Mar 24 14:01:32 EST 1997


Albert Spielmann <Albert.spielmann at bota.unine.ch> writes:

>I'm working with the two-hybrid system from Clontech.
>The yeast strains are CG1945 and Y190. Both have a ura3-52 deletion.
>Normally they can't grow without uracil. But as said in the supplier's
>protocol, it must grow!! Why?

	Your question is a bit ambiguous, under what conditions must the
strains grow?  I'm not familiar with the Clonetech system, but I would
wager it's to select for a URA3 marker on a plasmid...

	-Hernan



More information about the Yeast mailing list