Does anyone know about a plasmid such as pLG699-Z with a LEU marker? pLG699-Z carries the URA3 marker, the LacZ reporter and CYC leader sequences in addition to two XhoI sites for cloning of the promoter under study. I would really appreciate any comments on this. Thanks Monica Ruiz-Noriega Susan Henry's Lab email: mr6h+ at andrew.cmu.edu