DNA extraction from yeast - need help
cve at abc.univie.ac.at
Thu May 8 14:38:53 EST 1997
Magdalena Cano Plewinska wrote:
> He is trying to isolate yeast DNA and has had no luck. I gather that
> he is using glass beads to break up the cells and phenol for DNA
I think that the glass beads will
a) break the chromosomal DNA and
b) bind many DNA.
Most people use Zymolyase digestion of the cell wall. I can post you the
protocol if you want me to.
-- This is a Yeasts world ... yeah!
Christian Velten - actually guest at the Vienna Biocenter
Institute for Biochemistry and Molecular Cell Biology
Dr.-Bohrgasse 9 1030 Wien, Austria
Tel.: +43-1-795155506 Fax: +43-1-7995272
e-mail: cve at abc.univie.ac.at CIS: 10073,2071
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