One-hybrid false positives

Ralf Weigel weigeler at
Sat Oct 4 18:38:41 EST 1997

Dear Dr. Aranda,

in Plant Journal 11 (6) 1997, 1237-1251 the group of Dr. Terry Thomas
used an optimized one-hybrid system to find some DC3 binding proteins.
A better HIS3 reporter plasmid "was prepared by replacing the HIS3 gene
of pRS315HIS with a more sensitive one" (whatever this means).

Agustin Aranda wrote:
> I am obtaining many false positives in my one-hybrid screening. Does
> anybody know any tips to avoid this? Thanks,
> Agustin Aranda

R. Weigel
General Virology
Institute of Genetics
University of Hohenheim
Emil Wolff Str.14
70599 Stuttgart
Tel.: +049/711/4592395
Fax: ~4592937

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