Labelling yeast in glutamine medium?

Fergus Doherty Fergus.Doherty at nottingham.ac.uk
Thu Oct 16 07:24:09 EST 1997


I want to label yeast with 35S-met, but in a good nitrogen source. I don't
want to use ammonium sulphate (because the sulphate will be preferntially
used to make methionine - yes?).  So could I use glutamine or glutamate, if
so what concentration should I use.  What about ammonium chloride?

TIA

-- 
Fergus Doherty,
Dept Biochemistry,
Nottingham University,

Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)



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