False positives?

R. Weigel weigeler at uni-hohenheim.de
Tue Sep 30 08:24:46 EST 1997

Dear Dr. Harton,

since in Y190 his3 and lacZ are under the same uas, your "positives" are
most likely false positives.


jonathan harton wrote:
> I have experienced an interesting problem in the course of performing a
> two-hybrid screen.  After initially isolating several clones in strain
> Y190 that grow well in the presence of 35mM 3AT or more I continue to
> see growth but have not been able to demonstrate the production of
> b-galactosidase.  Is anyone aware of interactions that promote
> transcription from the HIS3 gene but fail to activate LacZ?
> Thanks in advance,
> Jon Harton
> Microbiology
> University of NC-Chapel Hill
> jharton at med.unc.edu

R. Weigel
General Virology
Institute of Genetics
University of Hohenheim
Emil Wolff Str.14
70599 Stuttgart
Tel.: +049/711/4592395
Fax: ~4592937

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