Plasmid Isolation from Yeast

aidan weatherill aweather at noham.worf.molbiol.ox.ac.uk
Mon Apr 6 10:39:37 EST 1998


> How to isolate plasmid from yeast?

We do much the same as the previous poster, But instead of using a fancy kit
we just use a normal alkaline lysis prep (E.mail me if you want our prep)
then zap into E.coli by electroporation. This works well for us. If there is
a plasmid there it will sucked up by the E.coli.

regards

Aidan
aweather at worf.molbiol.ox.ac.uk





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