pNMT1 + His3 shuttle vector

Nancy A. Markley markley at acs.ucalgary.ca
Thu Apr 16 13:04:08 EST 1998


Dear Yeast netters,

I am looking for an S.pombe/E.coli shuttle vector that contains
the pNMT1 thiamine-repressible promoter, and a His3 marker. Would
anyone have such a vector or know where I may find one? We are
interested in doing a library screen using three different
markers, and very much need the His3/pNMT1 variety of vectors. We
have the pREP series of plasmids but they contain either Leu or
Ura.

Thanks in advance,

Nancy.

***********************************************************************
Nancy Markley, Ph.D.
The University of Calgary,
Cancer Biology Research Group,
Dept. Medical Biochemisty and Molecular Biology
markley at acs.ucalgary.ca
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