integration of GFP in the yeast genome

didier Fesquet dfesque at ns1.nimr.mrc.ac.uk
Sat Apr 18 11:46:10 EST 1998


hi, to visualize the subcellular location of a protein in the yeast 
Scerevisiae,  I plan to integrate a single copy of a fusion between my 
gene and GFPS65T. I could not find in the litterature visualisation of 
a GFP fusion expressed under its own promoter (so probably at low to 
medium level!) people usually use gal driven promoter!. If anybody has 
ever tried this kind of experiment , I would be happy to hear about 
it! success, failure!

thanks

didier




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