frozen competent yeast cells?
kirkpat at ?cc.umanitoba.ca?
Wed Apr 22 12:10:58 EST 1998
<Pine.A41.3.96a.980420145748.27274B-100000 at dante18.u.washington.edu>, "S.
Tan" <sltan at u.washington.edu> wrote:
> Has anyone tried to use the LiAc method for preparing competent cells that
> can be frozen and used when needed?
Dr. Dan Gietz at the University of Manitoba is working on it as we speak.
He gets 1 000 000 transformants per microgram DNA and will be publishing
soon. He uses a few tricks that differ from the normal transformation
protocal but it is similar. I'll post an update when I can.
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