Zeocin

Aidan Weatherill Aweather at worf.molbiol.ox.ac.uk
Sat Aug 1 09:28:48 EST 1998


Does anyone here have any experience using Zeocin (invetrogen) in an
analogous way as Kanamx in Saccharomyces cerevisiae?

Specifically what concentration do people use for selecting transformants,
and are there any special media that people use?

Our lab has tried rich (YEPD) media with conc of between 100 - 300 mg/L.
Nothing grew at 300mg, and colonies that grew at lower concentrations dont
check out as real transformants.

Any help would be GRATEFULLY received

Aidan Weatherill
Yeast Genetics
Oxford University




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