GFP in yeast

Alex Brands abbrands at artsci.wustl.edu
Tue Aug 4 18:44:11 EST 1998


On 4 Aug 1998, David Humair wrote:

> Hi netters,
> I try to transform yeast  with GFP5 from HAseloff! The fluorescence is
> good under a confocal microscope but there is also a bright yellow
> light, even in wt cells. Does somebody know what it is?
> And not all the cells are fluorescent, even if they are coming from the
> same clone...Any suggestion?

In ade2 yeast strains (the ones that look pink), the pink adenine
precursor accumulates in the vacuole, and it fluoresces very nicely.  If
you supplement the media with enough adenine, the precursor won't
accumulate.

Alex





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