35-S Methionine labelling yeast cells??

Adrian Bracken Brackena at tcd.ie
Sat Aug 8 08:23:00 EST 1998


Hi,
I am wondering if it is possible to 35-S Methionine label yeast cell 
proteins? I want to label cells, prepare extracts, immunoprecipitate a 
ribonuclear-protein complex and run the protein content out on an SDS 
gel. Because the protein content will not be detectable by 
silver-staining, I hope the Methinine labelled proteins will be 
visable after exposure to film. 
Do you know if this approach has been used before? If so, have you any 
recent publications on it?
Please, if you could mail me at brackena at tcd.ie it would be great, 

Thanks a million, Adrian Bracken




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