electroporation

Stuart Montgomery stuart.montgomery at wolfson.oxford.ac.uk
Wed Aug 12 05:45:14 EST 1998


You've more than likely got too much salt in with the DNA. Dilute the DNA,
or ethanol precipitate and resuspend in water. Also keep the cuvettes and
cells on ice and dry well before zapping.

smasse wrote in message <35CAF695.9430F748 at uic.edu>...
>Does anyone have an idea as to why I sometimes get a "pop" when using a
>Biorad electroporation setup?  It sometimes happens and can blow the
>sample right out of the cuvette.  It is rather loud and scary besides!
>





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