35-S Methionine labelling yeast cells?? S.pombe

Stuart Montgomery stuart.montgomery at wolfson.oxford.ac.uk
Wed Aug 12 05:45:50 EST 1998


Adrian Bracken wrote in message <35CB226A.6A6A at tcd.ie>...
>Hi,
>I am wondering if it is possible to 35-S Methionine label yeast cell
>proteins? I want to label cells, prepare extracts, immunoprecipitate a
>ribonuclear-protein complex and run the protein content out on an SDS
>gel. Because the protein content will not be detectable by
>silver-staining, I hope the Methinine labelled proteins will be
>visable after exposure to film.
>Do you know if this approach has been used before? If so, have you any
>recent publications on it?
>Please, if you could mail me at brackena at tcd.ie it would be great,
>
>Thanks a million, Adrian Bracken
>

I want to measure protein half life in S.pombe and have been told that you
can't use Methinine. Does anyone have any ideas?
Thanks
Stuart Montgomery








More information about the Yeast mailing list