first drug assay HELP!

polverari dave at arletty.mnhn.fr
Wed Dec 9 15:53:07 EST 1998


hi everybody,

	 
	I have a molecule to test on yeast. for the moment i put 20µm on
exponential yeast in order to see at 600nm the effect on the yeast
growth. it allowed me to see an inhibition of about 25 % of control
(yeast with no molecule) in four hours. But now i would like to know if
this inhibition is due to death cells. what methods should i use to find
a death cells amount.
i heard about CFU, flow cytometry...

	What is the best solution and can you give me any protocols?

	Thanks 


PS : Sorry for my english ... :-)

				David polverari
				laboratoire de biophysique
				Museum National d Histoire Naturelle
				43 rue cuvier 
				Paris cedex 05
				01 40 79 36 95






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