tetrad dissection

S L Forsburg nospamforsburg at salk.edu
Fri Dec 11 13:45:20 EST 1998


Carolyn J. Decker (cdecker at mail.wsu.edu)

> I am looking for advise on tetrad dissection tools. I have made several
> dissection tools for my Nikon dissection scope using a glass rod and a
> piece of fiber optic cable that I cut with a razor blade or scalpel. The
> tools pick up tetrads well but do not release them efficiently. The tips
> of the fiber optic cable are not perfectly flat so I may be losing
> tetrads/spores in crevices in the tip.
> 
> Are dissection tools commercially available?
> 
> Does anyone have advice on how to make tools with flat tips?

We also use a Nikon tetrad scope.  We purchased from Carl Singer a
needle holder that mounts on the existing mechanical linkage,
and several boxes of precut needles.  I accidentally broke the
 first needle I mounted, which I did not realize for a bit, because 
even broken,  *it still worked better* than hand-cut.  
Also, the needles last a good long time.  Definitely 
worth the investment for us!  In fact one of the students
now claims doing tetrads is like playing a video game....

I have no official connection with Singer Instruments, just
a satisfied user. 


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S L Forsburg, PhD  forsburg at salk.edu
Molecular Biology and Virology Lab          
The Salk Institute, La Jolla CA 
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