death of pombe at 37OC?

Ge Qiang li gxl29 at po.cwru.edu
Tue Dec 15 11:51:05 EST 1998


I was looking at dominant negative effects of a mutant in haploid cells
expressed under NMT control.  But when I grew the transformants at 30
and 37 degree centigrates, I found that all transformants including wt
control died at 37 when plated on B1 containing plates.  Can somebody
come up with a theory to explain this observation?





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