yeast/mammalian fusions

Deepthi E. Reddy deepthi at
Wed Feb 4 15:33:19 EST 1998

I would really appreciate it if someone can give me information on the

1) Protocols for yeast/mammalian fusions

2) In my experiments where I have fused yeast cells containing a yac
retrofitted with neo gene, ( yeast spheroplasts were fused with the
mammalian cells), I have obtained colonies which grow in media
containing G418 but do not contain the neo gene!! Can somebody give me
any tips on how to work around this problem?

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