sporulation and plasmid maintenance

Jean-Yves Masson J.Masson at icrf.icnet.uk
Wed Jan 7 13:44:41 EST 1998


Dear yeasters:

        I would like to complement the meiotic defect of a mutant by
transforming it with a plasmid overexpressing a protein. I would like to
know if the ADH promoter would permit overexpression in the sporulating
medium containing potassium acetate. If not, which plasmid and promoter
would be the best for this type of experiment ??? Any comments or
suggestions would be greatly appreciated.


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Jean-Yves Masson
Postdoctoral fellow
Imperial Cancer Research Fund
Clare Hall Laboratories
South Mimms
Hertsfordshire EN6 3LD
United Kingdom
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Phone  (44) 0171 269 3873
Fax    (44) 0171 269 3801
E-mail  J.Masson at icrf.icnet.uk 








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