Non-expressing YAC transgenes
tomlinson at pplros.demon.co.uk
Wed Jan 28 07:51:23 EST 1998
I have recently made 5 lines of transgenic mice with a 400kb human YAC
but the human
genes are not expressed, which is contrary to my expectations to say the
I now want to compare the transgenic array to the YAC and the YAC to
DNA to look for problems. Does anyone have a methodology of choice? I'm
thinking of partial digests to map the YAC followed by complete digests
compare it to the genomic DNAs, but I wonder if there is a
method out there....
How common is it to find YACs that don't express in transgenics? I
anything in the literature.
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