Homologous recombination question

Frederik Boernke boernke at nospam.ipk-gatersleben.de
Fri Jun 5 07:04:51 EST 1998

gmclab221 wrote:
> I am interested in preparing some clones to test in the yeast two
> hybrid. Subcloning our PCR products
> has become difficult so what I am hoping to do  is to prepare clones by
> homologous recombinantion thus eliminating the entire E.coli step. I was
> wondering if anyone out there has had experience doing this with the
> two-hybrid vectors and yeast strains or had any advice as  to the
> methodologies that they could share. I do have the paper by Ma, H. et al
> (1987) Gene Vol. 58 describing this. Thanks in advance.
> Nancy Ayers
> nayers at cctr.umkc.edu

Hi Nancy,

this method has just been described in:
Petermann R. et al.,
A recombination based method to rapidly assess specificity ot two-hybrid
clones in yeast. NAR, 1998, Vol. 26,No 9

Good luck


Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.de

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