binkley at genome.Stanford.edu
Fri Jun 19 22:29:20 EST 1998
Matt Benson wrote:
>I'm trying to electroporate plasmid DNA from +ves in yeast two hybrid
>into E. coli. The only problem is that it arcs every time.
[description of various things tried...]
Have you tried varying the settings on the electroporator?
I was getting a lot of arcing using the manufacturer's suggested
settings (25 uF, 200 ohms, 2.5 kV) and tried lowering the voltage.
At 1.0 kV I get no arcing, a decent pulse time (between 4 and 5 msec),
and, most importantly, good transformation efficiency.
(We have a BioRad Gene Pulser.)
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