EU funded MPhil/PhD Studentship

Fergus Doherty Fergus.Doherty at
Mon Jun 22 08:14:10 EST 1998

MPhil/PhD programme in Advanced Molecular Biology

School of Biomedical Sciences University of Nottingham

The School of Biomedical Sciences, Nottingham University offers an Master
of Philosophy (MPhil) course in Molecular Biology starting October 1998.
The two year MPhil course comprises a research project together with
training inBioinformatics, Biopolymer Synthesis and Analysis and generic
skills. Excellent laboratory and computing facilities are provided by the
School and the University.

There will be opportunities forthe majority of students to transfer to a
PhD (subject to satisfactory progress).

To obtain an MPhil students will submit a thesis based on their research
project which will be assessed by an external examiner. A list of project
titles is provided.

European Union funding is available for stipends (equal to the current MRC
postgraduate stipend) and tuition fees for 15 successful applicants who are
citizens of members states of the European Union (EU). Applications are
invited from outside the EU, however in this case successful applicants
must provide for their living expenses, and pay the University tuition

Requests for application forms or further details should be sent to:
Dr. F. Doherty, School of Biomedical Sciences, Molecular & Cell Biology
Section, Floor D, University Medical School,
Queen9s Medical Centre, Nottingham NG7 2UH

e-mail: Fergus.Doherty at

The following yeast-based project is available:

Protein ubiquitylation and down-regulation of transporters and receptors by
vacuolar proteolysis

(Supervisors: Dr. F. Doherty/Dr. M.L. Landon)

Proteins are marked for degradation in the cytosol by the 26S proteasome by
tagging with the samll protein ubiquitin. However, we and others have shown
that ubiquitylated proteins accumulates in the lysosomes of mammalian cells
and the vacuole of yeast. Cell surface proteins, including receptors, are
also targeted to the lysosome by ubiquitylation follwing ligand binding.
This project will investigate the connection between ubiquitylation of cell
surface proteins (including receptors and transporters) and
lysosomal/vacuolar degradation using yeast mutants defective in various
components of the ubiquitin system and vacuolar apparatus. In particular
the role of the ubiquitin protein ligase Rsp5p in the ubiquitylation of
cell surface proteins will be investigated using molecular genetics. In
addition, the possible role of the de-ubiquitylating enzyme, Ubp4, in
vacuolar biogenesis will be investigated.

Fergus Doherty,
Dept Biochemistry,
Nottingham University,

Fergus.Doherty at
0115 970 9366 (74-41366 internal)

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