Selenomethionine

Katjusa Brejc katjusa at nki.nl
Mon Jun 29 10:37:32 EST 1998



Hi,

we would like to incorporate selenomethionine instead of methionine into a
particular protein expressed in yeast Pichia pastoris. Since our yeast
strain is not a methionine auxotroph we would have to modified our
medium in order to inhibit methionine biosynthesis. 
I would be very grateful if somebody could help me and tell which amino
acids to add to inhibit Met synthesis.

Thanks in advance

Katjusa Brejc  




katjusa at nki.nl





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