FOA

aidan weatherill aweather at noham.worf.molbiol.ox.ac.uk
Tue Mar 10 08:44:30 EST 1998


----------
In article <6dl3rc$nqj at net.bio.net>, Michael Forte <forte at ohsu.EDU> wrote:


>Hi,
>
>I know this has been discussed here before, but can anyone give me some
>hints as to how to make selection on FOA plates more stringent.  Right
>now, the difference in growth on minimal plates of ura+ and ura- strains
>is minimal.  Are there tricks to make this difference more pronounced? 
>Thanks in advance.
>
>Mike Forte
>


In our lab we replicate plates to 4-5 "junk" plates before replicating to
FOA, the Idea is to get a monolayer of cells on the velvet before going to
FOA this seems to work OK.

Aidan

Remove "noham." to reply by E-mail






More information about the Yeast mailing list