Homologue recombination

"David.Humair at bota.unine.ch" at NEDCU4.UNINE.CH "David.Humair at bota.unine.ch" at NEDCU4.UNINE.CH
Wed Mar 18 07:54:05 EST 1998



Hi netters,
I want to introduce a new sequence in a plasmid by homologue
recombination (or gap repair). My problem is that I cannot cut my
plasmid at the site of insertion but  200bp before. Is it possible to do
that, I mean to have two homologue sequences that can recognize
something on the plasmid but without a gap between them?

Thank you for your help,

David Humair







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