Plasmid rescue

Frederik Boernke boernke at nospam.ipk-gatersleben.de
Fri Oct 9 07:36:43 EST 1998


Paul Nutten wrote:
> 
> Does anyone have a good, reliable protocol for recovering plasmid DNA from
> cerevisiae for transformation/amplification in E. coli? I have tried
> several glass-bead based protocols, all with limited success.
>         Cheers,
>         Paul Nutten.

Hi Paul,

try the method of Hoffmann and Winston (1987) Gene 57, 267-272 it always
worked in my hands. If you tried this one already, let us know what the
problems were.

Good luck
Ricky

******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de




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