Plasmid rescue

Frederik Boernke boernke at
Fri Oct 9 07:36:43 EST 1998

Paul Nutten wrote:
> Does anyone have a good, reliable protocol for recovering plasmid DNA from
> cerevisiae for transformation/amplification in E. coli? I have tried
> several glass-bead based protocols, all with limited success.
>         Cheers,
>         Paul Nutten.

Hi Paul,

try the method of Hoffmann and Winston (1987) Gene 57, 267-272 it always
worked in my hands. If you tried this one already, let us know what the
problems were.

Good luck


Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515

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